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  2. Onderzoeken van whole body primary cell culture
    National Center for Biotechnology Information
    Novel methods to establish whole-body primary cell cultures for the cnidarians Nematostella vectensis and Pocillopora damicornis
    Cell culture is a valuable tool used for growing and maintaining cells in a controlled ex vivo environment1. Primary cell cultures are a type of short-term cell culture where the cells being maintained are the same types that came directly from
    Auteur: James D. Now…
    Gepubliceerd: 2021
    Publication: Sci Rep. …
    PubMed
    Novel methods to establish whole-body primary cell cultures for the cnidarians Nematostella vectensis and Pocillopora damicornis
    Cnidarians are emerging model organisms for cell and molecular biology research. However, successful cell culture development has been challenging due to incomplete tissue dissociation and contamination. In this report, we developed and tested several
    Auteur: James D Now…
    Gepubliceerd: 2021/0…
    Keywords: National In…
    National Center for Biotechnology Information
    Isolation, culture and characterization of primary cell lines of human buccal mucosal fibroblasts: A combination of explant enzamytic technique
    Background: The cell culture technique has become a routine and a popular method for its wide applications in the field of cell biology and biotechnology and in medical research. Isolation of primary cells over the cancer cells is an essential component
    Auteur: Ritiha Patil, Alk…
    Gepubliceerd: Jan-Ap…
    Publication: J Oral Ma…
    National Center for Biotechnology Information
    A Beginner’s Guide to Cell Culture: Practical Advice for Preventing Needless Problems
    The cultivation of cells in a favorable artificial environment has become a versatile tool in cellular and molecular biology. Cultured primary cells and continuous cell lines are indispensable in investigations of basic, biomedical, and translation
    Auteur: Sabine Weiskir…
    Gepubliceerd: 2023/03
    Publication: Cells. 202…
  3. Nature
    https://www.nature.com/articles/s41598-021-83549-7

    Novel methods to establish whole-body primary cell cultures for …

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  4. National Center for Biotechnology Information
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7893170

    Novel methods to establish whole-body primary cell cultures for …

  5. Mensen vragen ook naar
    Can We culture primary cells from all tissues of cnidaria vectensis and Pocillopora Dami?
    However, successful cell culture development has been challenging due to incomplete tissue dissociation and contamination. In this report, we developed and tested several different methodologies to culture primary cells from all tissues of two species of Cnidaria: Nematostella vectensis and Pocillopora damicornis.

    Novel methods to establish whole-body primary cell cultures for the

    nature.com
    What is a primary cell culture?
    Cell culture is a valuable tool used for growing and maintaining cells in a controlled ex vivo environment 1. Primary cell cultures are a type of short-term cell culture where the cells being maintained are the same types that came directly from the tissue, resulting in a diverse mix of cell types 2.

    Novel methods to establish whole-body primary cell cultures for the

    nature.com
    What is a primary culture?
    Primary cultures consist of cells that have been freshly derived from a living organism and are maintained for growth in vitro. Primary cells can be categorized according to the genus from which they are isolated, as well as by species or tissue type.

    Primary Cell Culture Guide | ATCC

    atcc.org
    How are polyclonal cells cultured?
    For subsequent cell culture, aliquots of polyclonal cell population were harvested from the primary 24-well culture plate, diluted fivefold with growth medium containing plasmin, and dispensed into 96-well plates (100 μL/well). Cells in clumps (Fig. 5 a) proliferated with a doubling time of 2–3 days (Fig. 5 b–d).

    Establishing Sustainable Cell Lines of a Coral, Acropora tenuis - Springer

    link.springer.com
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