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- Site-directed mutagenesis (SDM) is a technique to introduce specific changes in a DNA plasmid1. New England Biolabs (NEB) offers a Q5 Site-Directed Mutagenesis Kit that uses Q5 Hot Start High-Fidelity DNA Polymerase and custom mutagenic primers to create substitutions, deletions and insertions in plasmid DNA in less than 2 hours23. The kit can be used for a wide variety of plasmids up to at least 14 kb in length23.Learn more:✕This summary was generated using AI based on multiple online sources. To view the original source information, use the "Learn more" links.SDM is an in vitro procedure that uses custom designed oligonucleotide primers to confer a desired mutation in a double-stranded DNA plasmid.www.neb.com/en/applications/cloning-and-syntheti…The Q5 Site-Directed Mutagenesis Kit allows rapid site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours. The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase and custom mutagenic primers to create substitutions, deletions and insertions for a wide variety of plasmids up to at least 14 kb in length.www.neb.com/en/tools-and-resources/video-library…The Q5 Site-Directed Mutagenesis Kit enables rapid, site-specific mutagenesis of double-stranded plasmid DNA in less than 2 hours (Figure 1). The kit utilizes the robust Q5 Hot Start High-Fidelity DNA Polymerase along with custom mutagenic primers to create insertions, deletions and substitutions in a wide variety of plasmids.www.neb.ca/E0554
Oligonucleotide-directed mutagenesis with M13 DNA
Genome-scale analysis of interactions between genetic ... - Nature
Recruitment of multi-segment genomic RNAs by Bluetongue virus …
Queuosine biosynthetic enzyme, QueE moonlights as a cell …
Identification and characterisation of functional Kir6.1‐containing …
Molecular Microbiology | Microbiology Journal | Wiley Online Library
Human pannexin 1 channel is not phosphorylated by Src tyrosine …
Efficient expansion and CRISPR-Cas9-mediated gene ... - Cell Press
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