TurboGFP :: Fluorescent Protein Database
WebTurboGFP is a basic (constitutively fluorescent) green fluorescent protein published in 2006, derived from Pontellina plumata. It is reported to be a rapidly-maturing dimer with moderate acid sensitivity.
TurboGFP Sequence and Map - SnapGene
WebThis recognition sequence is asymmetric, so ligating blunt ends generated by BsrBI will not always regenerate a BsrBI site. BsrBI is typically used at 37°C, but can be used at temperatures up to 50°C. Start (0) 0 sites. TurboGFP. 1 .. 699 = …
Evrogen TurboGFP: Summary
WebTurboGFP is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell and organelle labeling and tracking the promoter activity. Destabilized TurboGFP variant allows accurate analysis of rapid and/or transient events in gene regulation.
Evrogen TurboGFP: Detailed description
WebTurboGFP is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell and organelle labeling and tracking the promoter activity. Destabilized TurboGFP variant allows accurate analysis of rapid and/or transient events in gene regulation.
TurboGFP: Properties, Sequence, MW, origin | Proteintech Group
WebTurboGFP is a bright green fluorescent protein used to study protein function, localization and dynamics in cells. Nanobody based research tools allow reproducible biochemical analysis including mass spectrometry and enzyme activity measurements of TurboGFP fusion proteins. 1. What are the differences between TurboGFP and GFP.
A molecular roadmap for the emergence of early-embryonic-like ... - Nature
Web18 dec. 2017 · Two-cell-like (turboGFP +) cells maintained Oct4 mRNA levels and displayed high expression of Zscan4 transcripts and of the MT2_mm and MT2B/C long terminal repeat (LTR)-driven chimeric...
Fluorescence Reporters turboGFP and turboRFP for Promoter
Web12 jan. 2024 · Herein, the features of overexpression of genes of green (turboGFP) and red (turboRFP) fluorescent proteins in Escherichia coli under the control of the IPTG-regulated phage promoter T5 were investigated. Both proteins were mainly synthesized in the soluble form and had a significant cytotoxicity.
Chromophore pre-maturation for improved speed and …
Web22 jan. 2019 · However, establishing a split version of TurboGFP might be precarious as TurboGFP is more prone to aggregation compared to superfolder GFP, which was used as starting-point for the GFP 1–10 and ...
A generic strategy for CRISPR-Cas9-mediated gene tagging
Web17 dec. 2015 · Using NanoLuc or TurboGFP, we demonstrate that tagged cell lines can be obtained and used to monitor endogenous gene expression (NanoLuc) or subcellular localization (TurboGFP).
Water diffusion in and out of the β-barrel of GFP and the fast …
Web2 jan. 2012 · TurboGFP is a second generation fluorescent protein with significantly improved chromophore maturation kinetics. Based on the crystal structure of TurboGFP it was proposed that a water filled pore leading from the outside to the chromophore may facilitate water and/or oxygen diffusion to the chromophore, thereby speeding up its ...
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